Composite

Part:BBa_K4653121

Designed by: Tangkun Zhu   Group: iGEM23_SZU-China   (2023-10-10)


SacB promoter - SpoVG RBS - MazE/F - Fd Terminator

SacB promoter is a sucrose induced promoter from Bacillus subtilis. MazEF is one of the most common Toxin-antitoxin systems (TA system), belonging to the kid family of the Class II TA classification system, and was first identified in E. coli. While mazF is a stable toxic protein capable of non-specifically cutting intracellular RNA and causing cell death, MazE is an unstable antitoxin that antagonizes MazF and can be degraded by the ATP-dependent ClpPa serine protease in cells. mazE is less stable than mazF, so cells must be able to survive when mazE is consistently expressed. And we needed a terminator to help us stop the above transcription, so we chose the fd terminator, which is the central terminator of bacteriophage fd. Additionally, we repeated the fd terminator as a strong terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 962
    Illegal AgeI site found at 682
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1231
    Illegal SapI.rc site found at 1175


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